A F-35B jet came down in South Carolina, the Marine Corps said. The pilot managed to safely eject and there were no injuries, BBC reports.
The Marines said in a statement that an investigation into the cause of the crash is underway.
The F-35 is the largest and most expensive weapons programme of its type in the world.
Global sales are projected at more that 3,000 aircraft, and the programme is likely to last for some 30 to 40 years.
But the programme has been criticised both for cost and combat effectiveness.
The jet involved in the incident is believed to have cost around $100m (£77m), although a new Pentagon contract announced on Friday for a record 141 F-35 jets has brought the cost down to around $89.2m per aircraft, according to Reuters.
The model is one of three varieties of the F-35 series in operation.
On Thursday, the US carried out its first operation using the F-35B against Taliban targets in Afghanistan, four months after the Israeli military announced it had used the F-35A to carry out two separate strikes.
* US President Trump has repeatedly praised the F-35, saying that the enemy “cannot see” it. While the jet is not invisible, its primary contractor Lockheed Martin has said its “advanced stealth” enables it to avoid radar.
* The fighter, first flown in 2006, was developed by Lockheed Martin as a versatile aircraft that could be used by the US Air Force, Marine Corps and Navy
* It has three variants: conventional takeoff and landing (A); short takeoff and vertical landing (B) and carrier-based catapult (C)
* Stealth is clearly a key factor, its airframe and design materials allow pilots to penetrate areas without being detected by radar
* This gives it a small radar cross-section, which is said to allow it to engage enemy aircraft before they see it. A helmet-mounted display system means the jet does not have to be pointing at its target to fire weapons
* But it’s the sensors, communications and avionics that it trumpets the most – data is shared immediately with operational commanders, and pilots can track the enemy, jam radars and thwart attacks